Purpose and Application
Extraction of Proteins from Mammalian cell or tissue cultures
2D electrophoresis sample buffer
Features
Separate extraction allows higher protein concentration per gel, spot detection is easy.
Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
Low running cost, while allowing short-time extraction and easy sample preparation
Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis
 
Specifications
 
AE-1435 EzApply 2D Kit
Major components
Wash buffer: Tris, NaCl
Solution 1: Tris, Ampholine, DTT
Solution 2: Urea, Thiourea, Surfactant
Alkylated reagent: Ampholine, DTT
Stratified solutions: Acrylamide, BPB, Urea
Package
Solution/30 mL bottle x 2 (Wash buffer)
Solution/20 mL bottle (Solution 1)
Solution/10 mL bottle (Solution 2)
Solution/1 mL tube (Alkylated reagent)
Solution/500 μL tube (Stratified solution)
Powder/tube (DTT)
Preparation
Appropriated amount of solution is applied to tissues or cells, which were lysed and extracted
Applicable amount
Extracted from 100 mg of mammalian tissue 20 runs
Storage
6 months at -20℃ (before dissolved in DTT)
1 week after dissolved in DTT
Ordering Information
Code No.
Description
Unit
2332335
AE-1435 EzApply 2D Kit
1 pk