Precast Gel I Agarose gel for IEF
agarGEL
Purpose and Application
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2D gel electrophoresis of protein samples
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an agarose gel for isoelectric focusing (the first dimension of 2D)
Features
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Ready to use
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A plenty volume of protein samples can be applied and separated
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Protein samples of high molecular weight (100 ~ 250 kDa) can also be separated.
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The gel can be easily removed from the column after electrophoresis
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A high-voltage power source is unnecessary. OK with constant voltage 300V, 150-210 min.
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Approximate separation pattern can be confirmed during fixation of the gel after electrophoresis
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agarGEL can be stored for up to 1 month at -20℃ after 1st electrophoresis and then 2nd electrophoresis
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Compatible electrophoresis apparatus: ATTO 2D Electrophoresis System (WSE-1510 DiscRun Ace)
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1-DE: agarGEL pH 5 to 8
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2-DE: e-PAGEL 12.5% (2D electrophoresis)
Description
※ The second dimension gel electrophoresis of agarGEL is suitable for the gel (PAGEL) which has a flat surface gel top without sample wells.
The second dimension gel (the front view)
Top of the gels of c-PAGEL (CD7.5/10/12.5/15/520L) and e-PAGEL (E-D7.5/10/12.5/520L) are flat without wells
The second dimension gel (the side view)
Put agarGEL (yellow) on the top of the second dimension gel (gray), and seal it tightly with agarose (light gray).
The glass plate of the second dimension gel is shown in light blue.
Chemiluminescence Imaging System
Centrifuse / Block Incubator / Others
